No Significant Difference between Plasma miRNAs and Plasma-Derived Exosomal miRNAs from Healthy People.

ABSTRACT:

Background

MicroRNAs (miRNAs) are small noncoding RNAs that are involved in many biological regulation processes. Studies have reported that miRNAs are enriched in human plasma and plasma-derived exosome as novel diagnostic biomarkers. The aim of this study was to determine whether the miRNA expression levels are different between plasma and plasma-derived exosome.

Methods

We sequenced and quantified the miRNAs in plasma and exosome from healthy blood samples and validated three miRNAs in the two groups of lung cancer samples by qRT-PCR.

Results

The sequencing results showed that only several of miRNAs were differential, while the qRT-PCR further validated that most of them did not have the consistent differences. However, the levels of two upregulated miRNAs (miR-181

SUBMITTER: Tian F 

PROVIDER: S-EPMC5471588 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature