Glucocorticoid receptor expression on circulating leukocytes differs between healthy male and female adults.

ABSTRACT:

Introduction

The glucocorticoid receptor (GR) is a key receptor involved in inflammatory responses and is influenced by sex steroids. This study measured GR expression on circulating leukocyte subtypes in males and females.

Methods

A total of 23 healthy adults (12 female) participated in this study. GR expression was measured in leukocyte subtypes using flow cytometry. Peripheral blood mononuclear cell (PBMC) gene expression of GR (NR3C1), GR β, TGF-β1 and 2, and glucocorticoid-induced leucine zipper (GILZ) were determined by real-time polymerase chain reaction.

Results

Leukocyte GR was lower in females, particularly in granulocytes, natural killer cells, and peripheral blood mononuclear cells (p≤0.01). GR protein expression was different across leukocyte subty

SUBMITTER: Lu KD 

PROVIDER: S-EPMC5471823 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature