Development of PCRSeqTyping-a novel molecular assay for typing of Streptococcus pneumoniae.

ABSTRACT:

Background

Precise serotyping of pneumococci is essential for vaccine development, to better understand the pathogenicity and trends of drug resistance. Currently used conventional and molecular methods of serotyping are expensive and time-consuming, with limited coverage of serotypes. An accurate and rapid serotyping method with complete coverage of serotypes is an urgent necessity. This study describes the development and application of a novel technology that addresses this need.

Methods

Polymerase chain reaction (PCR) was performed, targeting 1061 bp cpsB region, and the amplicon was subjected to sequencing. The sequence data was analyzed using the National Centre for Biotechnology Information database. For homologous strains, a second round of PCR, sequencing, and data

SUBMITTER: Nagaraj G 

PROVIDER: S-EPMC5471960 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature