C-Kit modifies the inflammatory status of smooth muscle cells.

ABSTRACT:

Background

c-Kit is a receptor tyrosine kinase present in multiple cell types, including vascular smooth muscle cells (SMC). However, little is known about how c-Kit influences SMC biology and vascular pathogenesis.

Methods

High-throughput microarray assays and in silico pathway analysis were used to identify differentially expressed genes between primary c-Kit deficient (Kit^W/W-v) and control (Kit^+/+) SMC. Quantitative real-time RT-PCR and functional assays further confirmed the differences in gene expression and pro-inflammatory pathway regulation between both SMC populations.

Results

The microarray analysis revealed elevated NF-κB gene expression secondary to the loss of c-Kit that affects both the canonical and alternative NF-κB pathwa

SUBMITTER: Song L 

PROVIDER: S-EPMC5472039 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature