Quantitative proteomics analysis of cartilage response to mechanical injury and cytokine treatment.

ABSTRACT: Mechanical damage at the time of joint injury and the ensuing inflammatory response associated with elevated levels of pro-inflammatory cytokines in the synovial fluid, are reported to contribute to the progression to osteoarthritis after injury. In this exploratory study, we used a targeted proteomics approach to follow the progression of matrix degradation in response to mechanical damage and cytokine treatment of human knee cartilage explants, and thereby to study potential molecular biomarkers. This proteomics approach allowed us to unambiguously identify and quantify multiple peptides and proteins in the cartilage medium and explants upon treatment with ±injurious compression ±cytokines, treatments that mimic the earliest events in post-traumatic OA. We followed degradation of differe

SUBMITTER: Wang Y 

PROVIDER: S-EPMC5472506 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature