Biodistribution and residence time of adenovector serotype 5 in normal and immunodeficient mice and rats detected with bioluminescent imaging.

ABSTRACT: As concerns increase about adenovirus type 5 (Ad5) being a safe gene transfer vector, it is important to evaluate its distribution, residence time, and possible toxicity in immunodeficient populations. To characterize the potential risk associated with different Ad5 vector delivery modes, we used immunocompetent and immunodeficient Rag2 ^-/- animals to establish mouse and rat models that could be monitored with bioluminescent imaging following intramuscular or intravascular infection with an engineered replication-incompetent Ad5 virus carrying the firefly luciferase gene (Ad5-Fluc). The Ad5 vector was less well-tolerated by Rag2 ^-/- animals than by wildtype ones, with delayed residence time, wider virus dissemination, less weight gain, and relatively severe pathologic

SUBMITTER: Liu Q 

PROVIDER: S-EPMC5472566 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature