TGF-beta/atRA-induced Tregs express a selected set of microRNAs involved in the repression of transcripts related to Th17 differentiation.

ABSTRACT: Regulatory T cells (Tregs) are essential regulators of immune tolerance. atRA and TGF-β can inhibit the polarization of naïve T cells into inflammatory Th17 cells, favoring the generation of stable iTregs, however the regulatory mechanisms involved are not fully understood. In this context, the roles of individual microRNAs in Tregs are largely unexplored. Naïve T cells were immunomagnetically isolated from umbilical cord blood and activated with anti-human CD2/CD3/CD28 beads in the presence of IL-2 alone (CD4_Med) or with the addition of TGF-β and atRA (CD4_TGF/atRA). As compared to CD4_Med, the CD4_TGF/atRA condition allowed the generation of highly suppressive CD4⁺CD25^hiCD127^-FOXP3^hi iTregs. Microarray p

SUBMITTER: Schiavinato JLDS 

PROVIDER: S-EPMC5472579 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature