An evolutionary optimization of a rhodopsin-based phototrophic metabolism in Escherichia coli.

ABSTRACT:

Background

The expression of the Gloeobacter rhodopsin (GR) in a chemotrophic Escherichia coli enables the light-driven phototrophic energy generation. Adaptive laboratory evolution has been used for acquiring desired phenotype of microbial cells and for the elucidation of basic mechanism of molecular evolution. To develop an optimized strain for the artificially acquired phototrophic metabolism, an ancestral E. coli expressing GR was adaptively evolved in a chemostat reactor with constant illumination and limited glucose conditions. This study was emphasized at an unexpected genomic mutation contributed to the improvement of microbial performance.

Results

During the chemostat culture, increase of cell size was observed, which were distinguished from that of the typical rod-

SUBMITTER: Kim HA 

PROVIDER: S-EPMC5472908 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature