Integrative analysis of DNA methylation, mRNAs, and small RNAs during maize embryo dedifferentiation.

ABSTRACT:

Background

Maize (Zea mays) is an important model crop for transgenic studies. However, genetic transformation of maize requires embryonic calli derived from immature embryo, and the impact of utilizing tissue culture methods on the maize epigenome is poorly understood. Here, we generated whole-genome MeDIP-seq data examining DNA methylation in dedifferentiated and normal immature maize embryos.

Results

We observed that most of the dedifferentiated embryos exhibited a methylation increase compared to normal embryos. Increased methylation at promoters was associated with down-regulated protein-coding gene expression; however, the correlation was not strong. Analysis of the callus and immature embryos indicated that the methylation increase was induced during induction of embr

SUBMITTER: Liu H 

PROVIDER: S-EPMC5472921 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature