Down-Regulation of CD62L Shedding in T Cells by CD39⁺ Regulatory T Cells Leads to Defective Sensitization in Contact Hypersensitivity Reactions.

ABSTRACT: Injection of regulatory T cells (Tregs) followed by sensitization with 2,4,6-trinitrochlorobenzene induced a transient increase in size and cellularity of skin-draining lymph nodes (LNs) in mice. This led us to hypothesize that Tregs may affect the trafficking of T cells from and to peripheral LNs. Two to three hours after sensitization, we found fewer CD8⁺ T cells expressing CD62L in LNs compared with untreated controls. Injection of wild-type Tregs prevented this down-regulation of CD62L. In contrast, Tregs devoid of the adenosine triphosphate (ATP)-degrading ecto-enzyme CD39 were unable to do so. As for the mechanism of CD62L regulation, we found that ATP, which is released in skin upon hapten-exposure, is inducing the protease ADAM17 in LN-residing T cells via engagement of

SUBMITTER: Mahnke K 

PROVIDER: S-EPMC5472987 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature