Fibronectin promotes directional persistence in fibroblast migration through interactions with both its cell-binding and heparin-binding domains.

ABSTRACT: The precise mechanisms through which insoluble, cell-adhesive ligands induce and regulate directional cell migration remain obscure. We recently demonstrated that elevated surface density of physically adsorbed plasma fibronectin (FN) promotes high directional persistence in fibroblast migration. While cell-FN association through integrins α₅β₁ and α_vβ₃ was necessary, substrates that selectively engaged these integrins did not support the phenotype. We here show that high directional persistence necessitates a combination of the cell-binding and C-terminal heparin-binding domains of FN, but does not require the engagement of syndecan-4 or integrin α₄β₁. FN treatment with various fixation agents indicated that associated chan

SUBMITTER: Missirlis D 

PROVIDER: S-EPMC5473823 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature