Dosage compensation in the process of inactivation/reactivation during both germ cell development and early embryogenesis in mouse.

ABSTRACT: Ohno proposed that dosage compensation in mammals evolved as a two-step mechanism involving X-inactivation and X-upregulation. While X-inactivation is well characterized, it remains to further analysis whether upregulation of the single activated X chromosome in mammals occurs. We obtained RNA-seq data, including single-cell RNA-seq data, from cells undergoing inactivation/reactivation in both germ cell development and early embryogenesis stages in mouse and calculated the X: A ratio from the gene expression. Our results showed that the X: A ratio is always 1, regardless of the number of X chromosomes being transcribed for expressed genes. Furthermore, the single-cell RNA-seq data across individual cells of mouse preimplantation embryos of mixed backgrounds indicated that strain-specific S

SUBMITTER: Li X 

PROVIDER: S-EPMC5473838 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature