A novel imaging method for correlating 2D light microscopic data and 3D volume data based on block-face imaging.

ABSTRACT: We have developed an imaging method designated as correlative light microscopy and block-face imaging (CoMBI), which contributes to improve the reliability of morphological analyses. This method can collect both the frozen sections and serial block-face images in a single specimen. The frozen section can be used for conventional light microscopic analysis to obtain 2-dimensional (2D) anatomical and molecular information, while serial block-face images can be used as 3-dimensional (3D) volume data for anatomical analysis. Thus, the sections maintain positional information in the specimen, and allows the correlation of 2D microscopic data and 3D volume data in a single specimen. The subjects can vary in size and type, and can cover most specimens encountered in biology. In addition, the requ

SUBMITTER: Tajika Y 

PROVIDER: S-EPMC5473845 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature