Noninvasive Measurement of mTORC1 Signaling with ⁸⁹Zr-Transferrin.

ABSTRACT: Purpose: mTOR regulates many normal physiological processes and when hyperactive can drive numerous cancers and human diseases. However, it is very challenging to detect and quantify mTOR signaling noninvasively in clinically relevant animal models of disease or man. We hypothesized that a nuclear imaging tool measuring intracellular mTOR activity could address this unmet need.Experimental Design: Although the biochemical activity of mTOR is not directly amenable to nuclear imaging probe development, we show that the transferrin receptor can be used to indirectly measure intracellular changes in mTOR activity.Results: After verifying that the uptake of radiolabeled transferrin (the soluble ligand of the transferrin receptor) is stimulated by active mTORC1 in vitro</

SUBMITTER: Truillet C 

PROVIDER: S-EPMC5474159 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature