Heterobifunctional Dyes: Highly Fluorescent Linkers Based on Cyanine Dyes.

ABSTRACT: Herein, we present a new synthetic route to cyanine-based heterobifunctional dyes and their application as fluorescent linkers between polymers and biomolecules. The synthesized compounds, designed in the visible spectral range, are equipped with two different reactive groups for highly selective conjugation under physiological conditions. By applying indolenine precursors with functionalized benzenes, we achieved water-soluble asymmetric cyanine dyes bearing maleimido and N-hydroxysuccinimidyl functionalities in a three-step synthesis. Spectroscopic characterization revealed good molar absorption coefficients and moderate fluorescence quantum yields. Further reaction with polyethylene glycol yielded dye-polymer conjugates that were subsequently coupled to the antibody cetuximab, of

SUBMITTER: Wycisk V 

PROVIDER: S-EPMC5474662 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature