Characterization of the scrambling domain of the TMEM16 family.

ABSTRACT: The TMEM16 protein family has 10 members, each of which carries 10 transmembrane segments. TMEM16A and 16B are Ca²⁺-activated Cl^- channels. Several other members, including TMEM16F, promote phospholipid scrambling between the inner and outer leaflets of a cell membrane in response to intracellular Ca²⁺ However, the mechanism by which TMEM16 proteins translocate phospholipids in plasma membranes remains elusive. Here we show that Ca²⁺-activated, TMEM16F-supported phospholipid scrambling proceeds at 4 °C. Similar to TMEM16F and 16E, seven TMEM16 family members were found to carry a domain (SCRD; scrambling domain) spanning the fourth and fifth transmembrane segments that conferred scrambling ability to TMEM16A. By introducing point mutations into T

SUBMITTER: Gyobu S 

PROVIDER: S-EPMC5474828 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature