A reference genome for Nicotiana tabacum enables map-based cloning of homeologous loci implicated in nitrogen utilization efficiency.

ABSTRACT:

Background

Tobacco (Nicotiana tabacum) is an important plant model system that has played a key role in the early development of molecular plant biology. The tobacco genome is large and its characterisation challenging because it is an allotetraploid, likely arising from hybridisation between diploid N. sylvestris and N. tomentosiformis ancestors. A draft assembly was recently published for N. tabacum, but because of the aforementioned genome complexities it was of limited utility due to a high level of fragmentation.

Results

Here we report an improved tobacco genome assembly, which, aided by the application of optical mapping, achieves an N₅₀ size of 2.17 Mb and enables anchoring of 64% of the genome to pseudomolecules; a significant increase from the previous va

SUBMITTER: Edwards KD 

PROVIDER: S-EPMC5474855 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature