Cystoviral RNA-directed RNA polymerases: Regulation of RNA synthesis on multiple time and length scales.

ABSTRACT: P2, an RNA-directed RNA polymerase (RdRP), is encoded on the largest of the three segments of the double-stranded RNA genome of cystoviruses. P2 performs the dual tasks of replication and transcription de novo on single-stranded RNA templates, and plays a critical role in the viral life-cycle. Work over the last few decades has yielded a wealth of biochemical and structural information on the functional regulation of P2, on its role in the spatiotemporal regulation of RNA synthesis and its variability across the Cystoviridae family. These range from atomic resolution snapshots of P2 trapped in functionally significant states, in complex with catalytic/structural metal ions, polynucleotide templates and substrate nucleoside triphosphates, to P2 in the context of viral capsids providing stru

SUBMITTER: Alphonse S 

PROVIDER: S-EPMC5476504 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature