Could monopronucleated ICSI zygotes be considered for transfer? Analysis through time-lapse monitoring and PGS.

ABSTRACT:

Purpose

The purpose of this study was to investigate the chromosomal constitution and the developmental potential of intracytoplasmic sperm injection (ICSI) deriving embryos displaying a single pronucleus at the zygote stage.

Methods

Eighty-eight embryos from single pronucleus (1PN) two polar bodies (2PB) ICSI zygotes from 64 preimplantational genetic screening (PGS) cycles (October 2012-December 2014), were retrospectively analyzed. Zygotes were cultured in a time-lapse incubator. Embryo biopsy was performed on day 3 and genetic analysis approached by array comparative genomic hybridization.

Results

Chromosomal analysis revealed that 17% (15/88) of embryos derived from 1PN 2PB zygotes were diagnosed as euploid. After blastomere biopsy at day 3, the blastocyst rate at

SUBMITTER: Mateo S 

PROVIDER: S-EPMC5476548 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature