Computational method allowing Hydrogen-Deuterium Exchange Mass Spectrometry at single amide Resolution.

ABSTRACT: Hydrogen-deuterium exchange (HDX) coupled with mass spectrometry (HDXMS) is a rapid and effective method for localizing and determining protein stability and dynamics. Localization is routinely limited to a peptide resolution of 5 to 20 amino acid residues. HDXMS data can contain information beyond that needed for defining protein stability at single amide resolution. Here we present a method for extracting this information from an HDX dataset to generate a HDXMS protein stability fingerprint. High resolution (HR)-HDXMS was applied to the analysis of a model protein of a spectrin tandem repeat that exemplified an intuitive stability profile based on the linkage of two triple helical repeats connected by a helical linker. The fingerprint recapitulated expected stability maximums and minimum

SUBMITTER: Gessner C 

PROVIDER: S-EPMC5476592 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature