Coupling optogenetic stimulation with NanoLuc-based luminescence (BRET) Ca⁺⁺ sensing.

ABSTRACT: Optogenetic techniques allow intracellular manipulation of Ca⁺⁺ by illumination of light-absorbing probe molecules such as channelrhodopsins and melanopsins. The consequences of optogenetic stimulation would optimally be recorded by non-invasive optical methods. However, most current optical methods for monitoring Ca⁺⁺ levels are based on fluorescence excitation that can cause unwanted stimulation of the optogenetic probe and other undesirable effects such as tissue autofluorescence. Luminescence is an alternate optical technology that avoids the problems associated with fluorescence. Using a new bright luciferase, we here develop a genetically encoded Ca⁺⁺ sensor that is ratiometric by virtue of bioluminescence resonance energy transfer (BRET). This sensor

SUBMITTER: Yang J 

PROVIDER: S-EPMC5476805 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature