Scanning superlens microscopy for non-invasive large field-of-view visible light nanoscale imaging.

ABSTRACT: Nanoscale correlation of structural information acquisition with specific-molecule identification provides new insight for studying rare subcellular events. To achieve this correlation, scanning electron microscopy has been combined with super-resolution fluorescent microscopy, despite its destructivity when acquiring biological structure information. Here we propose time-efficient non-invasive microsphere-based scanning superlens microscopy that enables the large-area observation of live-cell morphology or sub-membrane structures with sub-diffraction-limited resolution and is demonstrated by observing biological and non-biological objects. This microscopy operates in both non-invasive and contact modes with ∼200 times the acquisition efficiency of atomic force microscopy, which is achieve

SUBMITTER: Wang F 

PROVIDER: S-EPMC5476830 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature