Conjugating a groove-binding motif to an Ir(iii) complex for the enhancement of G-quadruplex probe behavior.

ABSTRACT: In this study, the reported G-quadruplex groove binder benzo[d,e]isoquinoline was linked to a cyclometallated Ir(iii) complex to generate a highly selective DNA probe 1 that retains the favorable photophysical properties of the parent complex. The linked complex 1 showed advantages of both parent complex 2 and groove binder 3. Similar to 3, the conjugated complex 1 exhibits a superior affinity and selectivity for G-quadruplex DNA over other conformations of DNA or proteins, with the fold enhancement ratio obviously improved compared with parent complex 2. The molecular modelling revealed a groove-binding mode between complex 1 and G-quadruplex. Meanwhile 1 also possesses the prominent advantages of transition meta

SUBMITTER: Wang M 

PROVIDER: S-EPMC5477052 | biostudies-literature | 2016 Apr

REPOSITORIES: biostudies-literature