A multiplex primer design algorithm for target amplification of continuous genomic regions.

ABSTRACT:

Background

Targeted Next Generation Sequencing (NGS) assays are cost-efficient and reliable alternatives to Sanger sequencing. For sequencing of very large set of genes, the target enrichment approach is suitable. However, for smaller genomic regions, the target amplification method is more efficient than both the target enrichment method and Sanger sequencing. The major difficulty of the target amplification method is the preparation of amplicons, regarding required time, equipment, and labor. Multiplex PCR (MPCR) is a good solution for the mentioned problems.

Results

We propose a novel method to design MPCR primers for a continuous genomic region, following the best practices of clinically reliable PCR design processes. On an experimental setup with 48 different combinatio

SUBMITTER: Ozturk AR 

PROVIDER: S-EPMC5477098 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature