Quantitative phosphoproteome on the silkworm (Bombyx mori) cells infected with baculovirus.

ABSTRACT:

Background

Bombyx mori has become an important model organism for many fundamental studies. Bombyx mori nucleopolyhedrovirus (BmNPV) is a significant pathogen to Bombyx mori, yet also an efficient vector for recombinant protein production. A previous study indicated that acetylation plays many vital roles in several cellular processes of Bombyx mori while global phosphorylation pattern upon BmNPV infection remains elusive.

Method

Employing tandem mass tag (TMT) labeling and phosphorylation affinity enrichment followed by high-resolution LC-MS/MS analysis and intensive bioinformatics analysis, the quantitative phosphoproteome in Bombyx mori cells infected by BmNPV at 24 hpi with an MOI of 10 was extensively examined.

Results

Totally, 6480 phosphorylation sites in 2112

SUBMITTER: Shobahah J 

PROVIDER: S-EPMC5477107 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature