IrCLIP platform for efficient characterization of protein-RNA interactions.

ABSTRACT: The complexity of transcriptome-wide protein-RNA interaction networks is incompletely understood. While emerging studies are greatly expanding the known universe of RNA-binding proteins, methods for the discovery and characterization of protein-RNA interactions remain resource intensive and technically challenging. Here we introduce a UV-C crosslinking and immunoprecipitation platform, irCLIP, which provides an ultraefficient, fast, and nonisotopic method for the detection of protein-RNA interactions using far less material than standard protocols.

SUBMITTER: Zarnegar BJ 

PROVIDER: S-EPMC5477425 | biostudies-literature | 2016 Jun

REPOSITORIES: biostudies-literature