In vitro labeling strategies for in cellulo fluorescence microscopy of single ribonucleoprotein machines.

ABSTRACT: RNA plays a fundamental, ubiquitous role as either substrate or functional component of many large cellular complexes-"molecular machines"-used to maintain and control the readout of genetic information, a functional landscape that we are only beginning to understand. The cellular mechanisms for the spatiotemporal organization of the plethora of RNAs involved in gene expression are particularly poorly understood. Intracellular single-molecule fluorescence microscopy provides a powerful emerging tool for probing the pertinent mechanistic parameters that govern cellular RNA functions, including those of protein coding messenger RNAs (mRNAs). Progress has been hampered, however, by the scarcity of efficient high-yield methods to fluorescently label RNA molecules without the need to drasticall

SUBMITTER: Custer TC 

PROVIDER: S-EPMC5477532 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature