The Role of Heterotypic DENV-specific CD8⁺T Lymphocytes in an Immunocompetent Mouse Model of Secondary Dengue Virus Infection.

ABSTRACT: Dengue is the most prevalent arthropod-borne viral disease worldwide and is caused by the four dengue virus serotypes (DENV-1-4). Sequential heterologous DENV infections can be associated with severe disease manifestations. Here, we present an immunocompetent mouse model of secondary DENV infection using non mouse-adapted DENV strains to investigate the pathogenesis of severe dengue disease. C57BL/6 mice infected sequentially with DENV-1 (strain Puerto Rico/94) and DENV-2 (strain Tonga/74) developed low platelet counts, internal hemorrhages, and increase of liver enzymes. Cross-reactive CD8⁺ T lymphocytes were found to be necessary and sufficient for signs of severe disease by adoptively transferring of DENV-1-immune CD8⁺T lymphocytes before DENV-2 challenge. Disease

SUBMITTER: Talarico LB 

PROVIDER: S-EPMC5478214 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature