The Microbiome of Infants Recruited to a Randomised Placebo-controlled Probiotic Trial (PiPS Trial).

ABSTRACT: The microbial dysbiosis associated with necrotizing enterocolitis (NEC) in preterm infants suggests that early exposure to probiotics may decrease and antibiotics may increase NEC risk. However, administration of Bifidobacterium breve strain BBG-001 to preterm infants did not affect NEC incidence in a multicenter randomised controlled phase 3 trial (PiPS trial). Using a subset of these subjects we compared the fecal microbiome of probiotic and placebo groups and assessed the impact of early antibiotic treatment. Extracted DNA from 103 fecal samples collected at 36weeks post-menstrual age underwent PCR amplification of a fragment of the 16S rRNA gene. Heatmaps were constructed showing the proportions of sequences from bacterial families present at >1% of the community. Stepwise logistic reg

SUBMITTER: Millar M 

PROVIDER: S-EPMC5478240 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature