PCR-RFLP of mitochondrial DNA reveals two origins of Apis mellifera in Taiwan.

ABSTRACT: Beekeeping has been a highly valued industry in Taiwan. As a result, many subspecies of Apis mellifera have been introduced to Taiwan since 1911, leading to the hybridization of different subspecies. In order to know the matrilineal origins of Taiwan A. mellifera, a total of 280 samples collected from 33 apiaries throughout the island were examined. Using PCR-RFLP of four mitochondrial gene fragments, i.e., the non-coding region between tRNA^leu and cytochrome c oxidase subunit II (intergenic tRNA^leu-COII), cytochrome b (Cyt b), large subunit rRNA (Ls rRNA) and cytochrome c oxidase subunit I (COI), we only found two haplotypes exist in 280 samples. Haplotypes ababa and bbbaa account for 87% of these Western bees belonged to the Eastern European (C)

SUBMITTER: Wu MC 

PROVIDER: S-EPMC5478285 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature