Differential Substrate Usage and Metabolic Fluxes in Francisella tularensis Subspecies holarctica and Francisella novicida.

ABSTRACT: Francisella tularensis is an intracellular pathogen for many animals causing the infectious disease, tularemia. Whereas F. tularensis subsp. holarctica is highly pathogenic for humans, F. novicida is almost avirulent for humans, but virulent for mice. In order to compare metabolic fluxes between these strains, we performed ¹³C-labeling experiments with F. tularensis subsp. holarctica wild type (beaver isolate), F. tularensis subsp. holarctica strain LVS, or F. novicida strain U112 in complex media containing either [U-¹³C₆]glucose, [1,2-¹³C₂]glucose, [U-¹³C₃]serine, or [U-¹³C₃]glycerol. GC/MS-based isotopolog profiling of amino ac

SUBMITTER: Chen F 

PROVIDER: S-EPMC5478678 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature