Relationship between retroviral DNA-integration-site selection and host cell transcription.

ABSTRACT: Retroviral DNA integration occurs throughout the genome; however, local "hot spots" exist where a strong preference for certain sites over others are seen, and more global preferences associated with genes have been reported. Previous data from our laboratory suggested that there are fewer integration events into a DNA template when it is undergoing active transcription than when it is not. Because these data were generated by using a stably transfected foreign gene that was only weakly inducible, we have extended this observation by comparing integration events into a highly inducible endogenous gene under both induced and uninduced transcriptional states. To examine the influence of transcription on site selection directly, we analyzed the frequency and distribution of integration of avi

SUBMITTER: Maxfield LF 

PROVIDER: S-EPMC547878 | biostudies-literature | 2005 Feb

REPOSITORIES: biostudies-literature