An Eulerian path approach to local multiple alignment for DNA sequences.

ABSTRACT: Expensive computation in handling a large number of sequences limits the application of local multiple sequence alignment. We present an Eulerian path approach to local multiple alignment for DNA sequences. The computational time and memory usage of this approach is approximately linear to the total size of sequences analyzed; hence, it can handle thousands of sequences or millions of letters simultaneously. By constructing a De Bruijn graph, most of the conserved segments are amplified as heavy Eulerian paths in the graph, and the original patterns distributed in sequences are recovered even if they do not exist in any single sequence. This approach can accurately detect unknown conserved regions, for both short and long, conserved and degenerate patterns. We further present a Poisson heu

SUBMITTER: Zhang Y 

PROVIDER: S-EPMC547885 | biostudies-literature | 2005 Feb

REPOSITORIES: biostudies-literature