Mushroom Emergence Detected by Combining Spore Trapping with Molecular Techniques.

ABSTRACT: Obtaining reliable and representative mushroom production data requires time-consuming sampling schemes. In this paper, we assessed a simple methodology to detect mushroom emergence by trapping the fungal spores of the fruiting body community in plots where mushroom production was determined weekly. We compared the performance of filter paper traps with that of funnel traps and combined these spore trapping methods with species-specific quantitative real-time PCR and Illumina MiSeq to determine the spore abundance. Significantly more MiSeq proportional reads were generated for both ectomycorrhizal and saprotrophic fungal species using filter traps than were obtained using funnel traps. The spores of 37 fungal species that produced fruiting bodies in the study plots were identified. Spore c

SUBMITTER: Castano C 

PROVIDER: S-EPMC5478987 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature