Hinderin, a five-domains protein including coiled-coil motifs that binds to SMC3.

ABSTRACT:

Background

The structural maintenance of chromosome proteins SMC1 and SMC3 play an important role in the maintenance of chromosomal integrity by preventing the premature separation of the sister chromatids at the onset of anaphase. The two proteins are constitutive components of the multimeric complex cohesin and form dimers by interacting at their central globular regions.

Results

In order to identify proteins that by binding to SMC3 may interfere with the protein dimerization process, a human cDNA library was screened by the yeast two-hybrid system by using the hinge region of SMC3 as bait. This has lead to the identification of Hinderin, a novel five domains protein including two coiled-coil motifs and sharing a strikingly structural similarity to the SMC family of protei

SUBMITTER: Patel CA 

PROVIDER: S-EPMC547899 | biostudies-literature | 2005 Jan

REPOSITORIES: biostudies-literature