Electric-Field-Induced Protein Translocation via a Conformational Transition in SecDF: An MD Study.

ABSTRACT: SecDF is an important component of the Sec protein translocation machinery embedded in the bacterial membrane, which is associated with many functions, such as stabilizing other Sec translocon components within the membrane, maintaining the transmembrane (TM) potential, and facilitating the ATP-independent stage of the translocation mechanism. Related studies suggest that SecDF undergoes functionally important conformational changes that involve mainly its P1-head domain and that these changes are coupled with the proton motive force (Δp). However, there still is not a clear understanding of how SecDF functions, its exact role in the translocation machinery, and how its function is related to Δp. Here, using all-atom molecular dynamics simulations combined with umbrella sampling, we study

SUBMITTER: Ficici E 

PROVIDER: S-EPMC5479055 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature