Flexible Fitting of Atomic Models into Cryo-EM Density Maps Guided by Helix Correspondences.

ABSTRACT: Although electron cryo-microscopy (cryo-EM) has recently achieved resolutions of better than 3 Å, at which point molecular modeling can be done directly from the density map, analysis and annotation of a cryo-EM density map still primarily rely on fitting atomic or homology models to the density map. In this article, we present, to our knowledge, a new method for flexible fitting of known or modeled protein structures into cryo-EM density maps. Unlike existing methods that are guided by local density gradients, our method is guided by correspondences between the α-helices in the density map and model, and does not require an initial rigid-body fitting step. Compared with current methods on both simulated and experimental density maps, our method not only achieves greater accuracy for prote

SUBMITTER: Dou H 

PROVIDER: S-EPMC5479111 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature