Prp40 Homolog A Is a Novel Centrin Target.

ABSTRACT: Pre-mRNA processing protein 40 (Prp40) is a nuclear protein that has a role in pre-mRNA splicing. Prp40 possesses two leucine-rich nuclear export signals, but little is known about the function of Prp40 in the export process. Another protein that has a role in protein export is centrin, a member of the EF-hand superfamily of Ca²⁺-binding proteins. Prp40 was found to be a centrin target by yeast-two-hybrid screening using both Homo sapiens centrin 2 (Hscen2) and Chlamydomonas reinhardtii centrin (Crcen). We identified a centrin-binding site within H. sapiens Prp40 homolog A (HsPrp40A), which contains a hydrophobic triad W₁L₄L₈ that is known to be important in the interaction with centrin. This centrin-binding site is highly conserved within the fi

SUBMITTER: Diaz Casas A 

PROVIDER: S-EPMC5479147 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature