M⁶A RNA Methylation Regulates the Self-Renewal and Tumorigenesis of Glioblastoma Stem Cells.

ABSTRACT: RNA modifications play critical roles in important biological processes. However, the functions of N⁶-methyladenosine (m⁶A) mRNA modification in cancer biology and cancer stem cells remain largely unknown. Here, we show that m⁶A mRNA modification is critical for glioblastoma stem cell (GSC) self-renewal and tumorigenesis. Knockdown of METTL3 or METTL14, key components of the RNA methyltransferase complex, dramatically promotes human GSC growth, self-renewal, and tumorigenesis. In contrast, overexpression of METTL3 or inhibition of the RNA demethylase FTO suppresses GSC growth and self-renewal. Moreover, inhibition of FTO suppresses tumor progression and prolongs lifespan of GSC-grafted mice substantially. m⁶A sequencing reveals that knockdown of

SUBMITTER: Cui Q 

PROVIDER: S-EPMC5479356 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature