YC-1 induces G₀/G₁ phase arrest and mitochondria-dependent apoptosis in cisplatin-resistant human oral cancer CAR cells.

ABSTRACT: Oral cancer is a serious and fatal disease. Cisplatin is the first line of chemotherapeutic agent for oral cancer therapy. However, the development of drug resistance and severe side effects cause tremendous problems clinically. In this study, we investigated the pharmacologic mechanisms of YC-1 on cisplatin-resistant human oral cancer cell line, CAR. Our results indicated that YC-1 induced a concentration-dependent and time-dependent decrease in viability of CAR cells analyzed by MTT assay. Real-time image analysis of CAR cells by IncuCyte™ Kinetic Live Cell Imaging System demonstrated that YC-1 inhibited cell proliferation and reduced cell confluence in a time-dependent manner. Results from flow cytometric analysis revealed that YC-1 promoted G₀/G₁ phase arrest and

SUBMITTER: Lee MR 

PROVIDER: S-EPMC5479426 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature