Improved metrics for comparing structures of macromolecular assemblies determined by 3D electron-microscopy.

ABSTRACT: Recent developments in 3-dimensional electron microcopy (3D-EM) techniques and a concomitant drive to look at complex molecular structures, have led to a rapid increase in the amount of volume data available for biomolecules. This creates a demand for better methods to analyse the data, including improved scores for comparison, classification and integration of data at different resolutions. To this end, we developed and evaluated a set of scoring functions that compare 3D-EM volumes. To test our scores we used a benchmark set of volume alignments derived from the Electron Microscopy Data Bank. We find that the performance of different scores vary with the map-type, resolution and the extent of overlap between volumes. Importantly, adding the overlap information to the local scoring functi

SUBMITTER: Joseph AP 

PROVIDER: S-EPMC5479444 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature