Sub-100-nm metafluorophores with digitally tunable optical properties self-assembled from DNA.

ABSTRACT: Fluorescence microscopy allows specific target detection down to the level of single molecules and has become an enabling tool in biological research. To transduce the biological information to an imageable signal, we have developed a variety of fluorescent probes, such as organic dyes or fluorescent proteins with different colors. Despite their success, a limitation on constructing small fluorescent probes is the lack of a general framework to achieve precise and programmable control of critical optical properties, such as color and brightness. To address this challenge, we introduce metafluorophores, which are constructed as DNA nanostructure-based fluorescent probes with digitally tunable optical properties. Each metafluorophore is composed of multiple organic fluorophores, organized in

SUBMITTER: Woehrstein JB 

PROVIDER: S-EPMC5479647 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature