Tissue Sources for Accurate Measurement of Germline DNA Genotypes in Prostate Cancer Patients Treated With Radical Prostatectomy.

ABSTRACT:

Background

Benign tissue from a tumor-containing organ is commonly the only available source for obtaining a patient's unmutated genome for use in cancer research. While it is critical to identify histologically normal tissue that is independent of the tumor lineage, few additional considerations are applied to the choice of this material for such measurements.

Methods

Normal formalin-fixed, paraffin-embedded seminal vesicle, and urethral tissues, in addition to whole blood, were collected from 31 prostate cancer patients having undergone radical prostatectomy. Genotype concordance was evaluated for DNA from each tissue source in relation to whole blood.

Results

Overall, there was a greater genotype call rate for DNA derived from urethral tissue (97.0%) in comparison

SUBMITTER: Emami NC 

PROVIDER: S-EPMC5479703 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature