De novo yeast genome assemblies from MinION, PacBio and MiSeq platforms.

ABSTRACT: Long-read sequencing technologies such as Pacific Biosciences and Oxford Nanopore MinION are capable of producing long sequencing reads with average fragment lengths of over 10,000 base-pairs and maximum lengths reaching 100,000 base- pairs. Compared with short reads, the assemblies obtained from long-read sequencing platforms have much higher contig continuity and genome completeness as long fragments are able to extend paths into problematic or repetitive regions. Many successful assembly applications of the Pacific Biosciences technology have been reported ranging from small bacterial genomes to large plant and animal genomes. Recently, genome assemblies using Oxford Nanopore MinION data have attracted much attention due to the portability and low cost of this novel sequencing instrumen

SUBMITTER: Giordano F 

PROVIDER: S-EPMC5479803 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature