Chromatographic studies of drug interactions with alpha₁-acid glycoprotein by ultrafast affinity extraction and peak profiling.

ABSTRACT: Interactions with serum proteins such as alpha₁-acid glycoprotein (AGP) can have a significant effect on the behavior and pharmacokinetics of drugs. Ultrafast affinity extraction and peak profiling were used with AGP microcolumns to examine these processes for several model drugs (i.e., chlorpromazine, disopyramide, imipramine, lidocaine, propranolol and verapamil). The association equilibrium constants measured for these drugs with soluble AGP by ultrafast affinity extraction were in the general range of 10⁴-10⁶M^-1 at pH 7.4 and 37°C and gave good agreement with literature values. Some of these values were dependent on the relative drug and protein concentrations that were present when using a single-site binding model; these results suggested a

SUBMITTER: Beeram S 

PROVIDER: S-EPMC5479935 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature