Chorioallantoic membranes of embryonated chicken eggs as an alternative system for isolation of equine influenza virus.

ABSTRACT:

Background

Influenza virus isolation in embryonated chicken eggs (ECEs) is not applicable for rapid diagnosis, however it allows the recovery and propagation of the viable virus. A low number of infectious virus particles in the swabs, poor quality of samples or individual strain properties can lead to difficulties during the virus isolation process. We propose to utilize chorioallantoic membranes (CAM) of ECEs with the assistance of real-time RT PCR to facilitate equine influenza virus isolation.

Methods

Real-time RT PCR was used to detect influenza virus genetic material in amniotic/allantoic fluids (AF) and CAM of ECEs. Haemagglutination assay was used for AF. We used highly diluted virus as a substitute of clinical specimen for ECEs inoculation.

Results

Our study

SUBMITTER: Gora IM 

PROVIDER: S-EPMC5480115 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature