Human lung and monocyte-derived macrophages differ with regard to the effects of β₂-adrenoceptor agonists on cytokine release.

ABSTRACT:

Background

β₂-adrenoceptor agonists have been shown to reduce the lipopolysaccharide (LPS)-induced cytokine release by human monocyte-derived macrophages (MDMs). We compare the expression of β₂-adrenoceptors and the inhibitory effect of formoterol and salmeterol on the LPS-induced release of tumor necrosis factor (TNF)-α, interleukin (IL)-1β, IL-6 and a range of chemokines (CCL2, 3, 4, and IL-8) by human lung macrophages (LMs) and MDMs.

Methods

LMs were isolated from patients undergoing resection and MDMs were obtained from blood monocytes in the presence of GM-CSF. LMs and MDMs were incubated in the absence or presence of formoterol or salmeterol prior to stimulation with LPS. The effects of formoterol were also assessed in the presence of the phosphod

SUBMITTER: Victoni T 

PROVIDER: S-EPMC5480184 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature