Metal Fluorides: Tools for Structural and Computational Analysis of Phosphoryl Transfer Enzymes.

ABSTRACT: The phosphoryl group, PO₃^-, is the dynamic structural unit in the biological chemistry of phosphorus. Its transfer from a donor to an acceptor atom, with oxygen much more prevalent than nitrogen, carbon, or sulfur, is at the core of a great majority of enzyme-catalyzed reactions involving phosphate esters, anhydrides, amidates, and phosphorothioates. The serendipitous discovery that the phosphoryl group could be labeled by "nuclear mutation," by substitution of PO₃^- by MgF₃^- or AlF₄^-, has underpinned the application of metal fluoride (MF _x ) complexes to mimic transition states for enzymatic phosphoryl transfer reactions, with sufficient stability for experimental analysis. Protein crystallog

SUBMITTER: Jin Y 

PROVIDER: S-EPMC5480424 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature