The C-Box Region of MAF1 Regulates Transcriptional Activity and Protein Stability.

ABSTRACT: MAF1 is a conserved negative regulator of RNA polymerase (pol) III and intracellular lipid homeostasis across species. Here, we show that the MAF1 C-box region negatively regulates its activity. Mutations in Caenorhabditis elegans mafr-1 that truncate the C-box retain the ability to inhibit the transcription of RNA pol III targets, reduce lipid biogenesis, and lower reproductive output. In human cells, C-box deletion of MAF1 leads to increased MAF1 nuclear localization and enhanced repression of ACC1 and FASN, but with impaired repression of RNA pol III targets. Surprisingly, C-box mutations render MAF1 insensitive to rapamycin, further defining a regulatory role for this region. Two MAF1 species, MAF1_L and MAF1_S, are regulated by the C-box YSY motif, which, when muta

SUBMITTER: Pradhan A 

PROVIDER: S-EPMC5480515 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature