High-Throughput Patch Clamp Screening in Human α6-Containing Nicotinic Acetylcholine Receptors.

ABSTRACT: Nicotine, the addictive component of tobacco products, is an agonist at nicotinic acetylcholine receptors (nAChRs) in the brain. The subtypes of nAChR are defined by their α- and β-subunit composition. The α6β2β3 nAChR subtype is expressed in terminals of dopaminergic neurons that project to the nucleus accumbens and striatum and modulate dopamine release in brain regions involved in nicotine addiction. Although subtype-dependent selectivity of nicotine is well documented, subtype-selective profiles of other tobacco product constituents are largely unknown and could be essential for understanding the addiction-related neurological effects of tobacco products. We describe the development and validation of a recombinant cell line expressing human α6/3β2β3^V273S nAChR for screening

SUBMITTER: Armstrong LC 

PROVIDER: S-EPMC5480602 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature